Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Download the SpectraMax Mini Reader Plug-in and associated guides. These lab workhorses are used in drug discovery. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. 0 out of 5 $ 5,999. 303 unable to cal dark current. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. To resume collecting kinetic measurements within the same file, press the Append button. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 4 μL DNA standards. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 304 signal level saturation. Find regional support. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Add to Cart. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. flexibility of monochromator-based systems. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. The calculated LOQ values were 0. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. Products : General, Microplate Readers. Microplate Readers BioTek Epoch 2 Microplate Reader. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. . SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The plate reader optics are tunable for. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Nuclear Receptor. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Step 4: Incubate at room temperature for 1 hour, protected from light. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 2 only: SpectraMax Mini Update. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). This unlocks the transport slide. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. View the Molecular Devices website. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax ® M2 Multi-Mode Microplate Reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SAN JOSE, CA — Molecular Devices, LLC. 2. For readers without a cuvette port, simply check the box next to ‘PathCheck’. This unlocks the transport slide. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Three low volume 24-well bottom slides. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. Molecular Devices Spectramax iD3 Microplate Reader. Step 1: Turn on your microplate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Access this by going to Help >. Contents of wells were pipetted up and down several times to mix. microplate reader. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Sensitive fluorescent quantitation of DNA with the Quant-iT. . 1. With optimized reagents, option to operate the. 0 out of 5 $ 23,995 $ 22,495. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. ATP standard curves read on SpectraMax microplate readers. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax Mini User Guide. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Figure 1. 100 readings per well gives optimal Z’ values for this assay. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. We would like to show you a description here but the site won’t allow us. Step 1: Open the SoftMax Pro IMAP FP protocol. Examples: “Press carefully to engage,” “ Do not. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 6% for the on-board optics (monochromators). . The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The plate reader can measure multiple readouts, including UV and visible absorbance. Bookings are free, but you will need to use iLab to book. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Microplate reader assays requiring infrared (IR) or red-shifted. 100 fmol 384-well (75 µL) 0. both cuvette-based and microplate reader applications on the same instrument. SpectraMax Microplate Readers. . The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. No additional manuals are available. . Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Temperature control and shaking come as standard. The guide also covers the software features, the specifications, and the accessories of the systems. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The plate reader can measure multiple readouts, including UV. Download PDF. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The plate reader optics are tunable for absorbance. Step 2: Launch SoftMax Pro Software. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. MPN:SpectraMax Plus. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. Summary of Contents for Molecular Devices SpectraMax M2. It features a modular design that allows users to add or change detection cartridges according to their needs. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. 0 out of 5 $ 6,499. , April 14, 2022 – Molecular Devices, LLC. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Contact our support team with your questions regarding Molecular Devices products and applications. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. With optimized reagents, option to operate the. 72 MB. . AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. flexibility of monochromator-based systems. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The following document is intended to demonstrate setup of this instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The University of Rouen in Normandy uses the SpectraMax iD3 and. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 1. 2. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax Spectrofluorometers. Here, the CVs for each plate were less than 3, and the difference between mean. Unlock your i3/i3x instrument after shipment. Detailed specifications of each model reader are reported in Section. SpectraMax M5 reader settings are shown. Endpoint, kinetic, spectrum. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The plate. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 7°C (gradient heating design). The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. 314 low lamp level warning. Results are shown in Table 1. Most papers they cultured bacteria to OD600>0. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Click SoftMax Pro Software User Guide. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Cuvette Microplate wells. begin reading microplates with seven mouse clicks. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Or scan up to 384 wells at any time to determine the best assay conditions. Page 28 Chapter 5: Read Modes and Read Types 1. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The 2-channel configuration is available with 0, 2, or 4 injectors. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. The drawing shows a standard microplate of 15 mm height covered with a lid. On the back, in the right hand top corner,. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. 306 plate air cal fail,low light. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. . The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Reader modes are customized to your specific applications with options to upgrade easily. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Unparalleled performance on a personalized platform. 7°C (gradient heating design). 3) At the bottom of the strip, click on "Show. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. 76358-624. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. . View the Molecular Devices website. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. It also integrates with the. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Fill a clean plate with water. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Lysozyme contains two tryptophan residues that. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. All stand-alone instrument functions can be controlled using the software. Products. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. 40266 Email: drugdiscoverytech@lifetech. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Italic and boldface type are used for emphasis. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® ABS Plus Microplate Reader can run . The system uses two scanning monochromators to determine the optimal excitation and emission settings. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Microplate Readers. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 out of 5 $ 11,999. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. begin reading microplates with seven mouse clicks. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. This pathlength correction is accomplished only with the use of. Published Dec 16, 2020 | Updated May 19, 2023. \ Figure 3. Positions H21-K22 contained plate blanks of PBS only. In addition, the SpectraMax iD5 can be expanded to. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Assays can be better optimized by scanning across. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Figure 1. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. no. The screw remains inside the instrument. Total volume (except deep. 1. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. When the user applies a set temperature of 37°C, the top is heated to 37. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. Microplate Readers Biotek Epoch Microplate Reader. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. In addition, the SpectraMax® iD5 can be expanded to include bottom. zip. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. e. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. Align the two slots on the bottle holder with the two pegs on the rail. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Highly customizable low volume. 997 (n = 3). The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. When the user applies a set temperature of 37°C, the top is heated to 37. 1. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. keeping with best practices, reader performance should. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Figure 3. With optimized reagents, option to operate the. Step 2: Launch SoftMax Pro Software. The advanced optical and. Multimode Microplate Reader. Turn the reader off by pulling the power cord. Figure 2-23: Bottle holder To install the bottle holder: 1. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. . Plate chamber design of the SpectraMax category 1 microplate readers. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. The dual monochromator optics allow the. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Devices’ StakMax® Microplate Stacker in a matter of minutes and . A: Microplate Preparation. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. 304 signal level saturation. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 2 only) SpectraMax M-series Microplate readers. Example: [Drawer]. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Results are shown in Table 1. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Devices’ StakMax® Microplate Stacker in a matter of minutes and . SpectraMax® Microplate Readers and SoftMax® Pro Software. Omnia® Compatible Microplate Reader Documentation Version No. 20 μL of Component A was added to each well of the microplate. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C.